10th amendment of FCO 1985

7346 GI/202 3 (1) EXTRAORDINARY PART II—Section 3—Sub-section ( ii) PUBLISHED BY AUTHORITY No. 4842] NEW DELHI, FRIDAY , NOVEMBER 24, 2023/ AGRAHAYANA 3, 1945 CG-DL-E-28112023-250283 (iii) पी एच (5 % घोल) 6.0 to 7.0 (iii) पी एच (5% घोल) 6.0 to 7.0 (iii) पी एच (5% घोल) 6.0 to 7.0 (iii) पी एच (5% घोल) 5.5-6.5 (ii) पी एच (1 % घोल) 5.5-6.5 MINISTRY OF AGRICULTURE AND FARMERS WELFARE (Department of Agriculture, and Farmers Welfare) ORDER New Delhi, the 23rd November , 2023 S.O. 5051(E).— In exercise of the powers conferred by section 3 of the Essential Commodities Act, 1955 (10 of 1955), the Central Government hereby makes the following Order further to amend the Fertiliser (Inorganic, Organic or Mixed) (Contr ol) Order, 1985, namely: - 1. This Order may be called the Fertiliser (Inorganic, Organic or Mixed) (Control) Tenth Amendment Order, 2023. (2) It shall come into force on the date of its publication in the Official Gazettee. 2. In the Fertiliser ( Inorganic, Organic or Mixed ) (Control) Order, 1985 ( hereinafter referred to as the said Order,) namely: - (I) in Schedule I, PART -A “ SPECIFICATIONS OF FERTILISERS ,- (a) in Sub -heading “1 (g) MICRONUTRIENTS,” after serial number 25 and entries relating there to, the following serial numbers and entries shall be inserted, namely: - “ 26 Chelated Calcium as Ca-EDTA (i) Appearance – Free flowing powder (ii) Calcium content (expressed as Ca), percent by weight minimum in the form of Ca-EDTA 9.0 (iii) pH (5 % solution) 6.0 - 7.0 (iv) Matter insoluble in water percent by weight (maximum.) 0.5 (v) Lead (as Pb) percent by weight , maximum 0.003 (vi) Cadmium (as Cd) percent by weight, maximum 0.0025 (vii) Arsenic (as As) percent by weight, maximum 0.01 27. Chelated Mag nesium as Mg-EDTA (i) Appearance – Free flowing powder (ii) Magnesium content (expressed as Mg), percent by weight minimum in the form of Mg-EDTA 5.0 (iii) pH (5 % solution) 6.0-7.0 (iv) Matter insoluble in water percent by weight (maximum) 0.5 (v) Lead (as Pb) percent by weight ,maximum 0.003 (vi) Cadmium (as Cd) percent by weight, maximum 0.0025 (vii) Arsenic (as As) percent by weight, maximum 0.01 28. Chelated Manganese as Mn-EDTA (i) Appearance – Free flowing powder (ii) Manganese content (expressed as Mn), percent by weight minimum in the form of Mn-EDTA 10.0 (iii) pH (5 % solution) 6.0-7.0 (iv) Matter insoluble in water percent by weight ,maximum 0.5 (v) Lead (as Pb) percent by weight ,maximum 0.003 (vi) Cadmium (as Cd) percent by weight, maxi mum 0.0025 (vii) Arsenic (as As) percent by weight, maximum 0.01 29. Chelated Copper as Cu-EDTA (i) Appearance – Free flowing powder (ii) Copper content (expressed as Cu), percent by weight minimum in the form of Cu-EDTA 12.0 (iii) pH (5 % solution) 5.5 – 6.5 (iv) Matter insoluble in water percent by weight (max.) 0.5 (v) Lead (as Pb) percent by weight , maximum 0.003 (vi) Cadmium (as Cd) percent by weight, maximum 0.0025 (vii) Arsenic (as As) percent by weight, maximum 0.01” (II) in sub -heading “1 (h) FORTIFIED FERTILISERS”, after serial number 34 and entries relating there to, the following serial numbers and entries shall be inserted, namely: - “35. Single Super Phosphate fortified with Zinc, Iron and Boron (i) Moisture percen t by weight, maximum 5.0 (ii) Available phosphorous (as P2O5) percent by weight, minimum 16.0 (iii) Water soluble phosphorous (as P2O5) per cent. by weight, minimum 14.5 (iv) Iron (as Fe) per cent. by weight , minimum 0.25 (v) Zinc (as Zn) percent by weight, minimum 0.5 (vi) Boron (as B) per cent. by weight, minimum 0.2 (vii) Free Phosphoric acid (as P2O5) percent. by weight, maximum 4.0 (viii) Sulphate Sulphur (as S) percent by weight, minimum 11.0 (ix) Particle size-Minimum 90 percent of the material shall be retained between through 1.0 mm and 4 mm IS sieve” (III) in sub-heading “1 (k) Liquid Fertiliser’’ after serial number 12 and entries relating there to , the following serial numbers and entries shall be inserted, namely; - 13. Zinc Gluconate (i) Zinc as ( Zn) per cent. by weight, minimum 12.0 (ii) pH (1% solution) 5.5-6.5 (iii) Specific gravity 1.28 -1.45 (iv) Lead(as Pb) per cent. by weight, maximum 0.003 (v)Cadmium (as CD) per cent. by weight , maximum 0.0025 (vi)Arsenic (as As) per cent. by weight , maximum 0.01 3. In Schedule II, “PART -B METHODS OF ANALYSIS OF FERTILISERS”, after serial Number 35 and entries relating thereto, the following serial numbe r and entries shall be inserted, namely: - ‘‘36. Method of analysis Chelated Manganese as Mn-EDTA: - A. Reagents: - (i) Glass distilled water or demineralized water of pH 2.5+/ - 0.5:-Dilute l ml of 10% H2SO4 to one litre with glass distilled water or demin eralized water and adjust the pH to 2.5 with a pH meter using 10% H2SO4 or NaOH. (ii) Di Sodium EDTA solution :- Dissolve 0.73 gm of di sodium EDTA di hydrate in distilled water and make volume to 100 ml. B. Preparation of standard Manganese metal stock solution [1000 ppm]: - (i) Dissolve 1.0 gm of Manganese metal in 50 ml of conc. Hydrochloric acid and make volume to 1000 ml with distilled water. This is 1000 ppm Manganese stock solution. (ii) Intermediate solution (100 ppm): - Pipette 10 ml of Manganese stock solution and 10 ml of Di sodium EDTA solution in 100 ml of volumetric flask and dilute to volume with distilled water. (Note: - In case the metal stock solution is not exactly 100.0 ppm, the exact titer value of di sodium EDTA required to chelate the metal has to be added.) (iii) Working Standard Solution :- Pipette following volumes of 100 ppm intermediate solution in 100 ml Volumetric flask and make volume with acidified water. Volume of 100 ppm intermediate solution taken To make volume to 100 ml Conce ntration of Mn in ppm After making vol. to 100 ml 1.0 ml 100 ml 1.0 ppm 2.0 m1 100 ml 2.0 ppm 3.0 ml 100 ml 3.0 ppm 4.0 ml 100 ml 4.0 ppm C. Preparation of sample solution for Chelated Manganese: - (i) Weigh 1.0 gm of sample and trans fer into 100 ml volumetric flask and make volume with distilled water. Pipette out 1.0 ml of sample solution and dilute further to make second dilution of 100 m1 with distilled water, pipette 5 ml from second dilution and make volume to 50 ml with acidified water to get the above flaming range from 1.0-4.0 ppm. (ii) Flaming the solutions: - Flame the standards and the sample of Chelated Manganese on atomic absorption spectrophotometer at a wavelength of 279.5 nm (Mn line of instrument ) using air-acetylene flame. (iii) Prepare a blank solution from which only the sample has been omitted. Flame along with standards and sample. D. Calculations: - Prepare a standard curve of 1‹nown concentration of Manganese solutions by plotting the absorbance value on Y-axis against their respective concentrations on X-axis. Part -I :- Determination of Total Manganese content in Chelated Manganese sample: - Total Mn content = Concentration of Mn in ppm obtained after flaming the solution X 10 Part-II :- Determination of Free Manganese content in Chelated sample: - Reagents: - 0.02 M Disodium salt of EDTA — 0.7444 gm of Disodium Salt of EDTA in 100 ml of distilled water. Standardize with 0.02 M standard Zinc or Manganese Metal solution. (i) Hydroxylamine Hydrochloride — 0.1-0.2 gm; (i) Trietanolamine — 1-2 m1; (ii) Buffer solution of pH — 10.0;and (iii) Indicator Eriochrome black T (EBT) powder. Take 10 ml of aliquot from stock dilution of sample (1.0 g in 100 ml) Add to it 0.1 g Hydroxylamine HCl, 1to 2 ml of triethanolamine, 10 ml buffer of pH 10.0, EBT indicator and titrate against 0.02 M disodium EDTA solution. % Free Manganese=Titration value of EDTA x Atomic weight of Mn metal Molarity of EDTA x 1 % Chelated Manganese content = % Total Manganese - % Free Manganese content 37. Method of analysis of Chelated Calcium as Ca EDTA: - A. Reagents: - (i) G1ass distilled water or demineralized water of pH 2.5+/ - 0.5:- Dilute l ml of 10% H2SO4 to one litre with glass distilled water or demineralized water and adjust the pH to 2.5 with a pH meter using 10% H2SO4 or NaOH. (ii) Di sodium EDTA solution: - Dissolve 0.73 gm of di sodium EDTA di hydrate in distilled water and make volume to 100 ml. (iii) Strontium Chloride (SrCl2 .6H2O )- 15.0 gm in 0.5N HCl and dilute to 100 ml. B. Preparation of Standard stock solution of Calcium 1000 ppm. - (i) Dissolve 2.498 gm CaCO3 in a minimum amount of 3 N HCl. Dilute to 1000 ml with distilled water. This is 1000 ppm calcium stock solution. (ii) Intermediate solution [100 ppm]. - Pipette 10 ml of Calcium stock solution and 50 ml of Diso dium EDTA solution in 100 m1 volumetric flask and dilute volume with distilled water. (Note: - In case the metal stock solution is not exactly 100.0ppm, the exact titer value of disodium EDTA required to chelate the metal has to be added.) (iii) Working Standard solution. - Pipette following volumes of 100 ppm intermediate solution in 100 ml Volumetric flask and make volume with acidifi ed water. Volume of 100 ppm intermediate solution taken Volume of strontium chloride To be added Concentration of Ca in ppm after making vo1. to 100 ml 5.0 ml 10 ml 5.0 ppm 10.0 ml 10 m1 10.0 ppm 15.0 ml 10 m1 15.0 ppm 20.0 ml 10 ml 20.0 ppm C. Preparation of sample solution for Chelated Calcium. - (i) Weigh 1.0 gm of sample and transfer into 100 ml volumetric flask and make volume with distilled water. Pipette out 5.0 ml of sample solution and dilute further to make second dilution of 100 ml with distilled water, pipette 10.0 ml from second dilution and make volume to 100 ml with acidified water to get the above flaming range from 5.0-20.0 ppm. (ii) Flaming the solutions. - Flame the standards and the sample of Chelated Calcium on Atomic Absorbption Spectrophotometer at a wavelength of 422.7 nm (Ca line of instrument) using air-acetylene flame. (iii) Prepare a blank solution from which only the sample has been omitted. Flame along with standards and sample. D. Calculations. - Prepare a standard curve of known concentration of Calcium solutions by plotting the absorbance value on Y-axis against their respective concentrations on X- axis. Part-I :- Determination of Total Calcium content in Chelated Calcium sample: - Total Ca content = Concentration of Ca in ppm obtained after flaining the solution X 2 Part -II :- Determination of Free Calcium in Chelated sample: - (i) Reagents: - 0.02 M Disodium salt of EDTA — 0.7444 gm of Disodium Salt of EDTA in 100 ml of distilled water. Standardize with 0.02 M standard Zinc and Manganese Metal solution. (ii) Buffer solution of pH — 10.0 (iii) Indicator Eriochrome black T (EBT) powder, - (iv) Take 10 ml of aliquot from stock dilution of sample (1.0 g in 100 ml) Add to it 0.1 g Hydroxylamine HCl, 1to 2 ml of triethanolamine, 10 ml buffer of pH 10.0, EBT indicator and titrate against 0.02 M disodium EDTA solution. % Free Calcium = Titration value of EDTA x Atomic weight of C a metal x Molarity of EDTA x 1 % Chelated Calcium content = % Total Calcium - % Free Calcium content 38. Method of analysis of Chelated Magnesium as Mg-EDTA: - A. Reagents. - (i) Glass distilled water or demineralized water of pH 2.5+/ - 0.5. - Dilute 1m1 of 10% H2SO4, to one litre with glass distilled water or demineralized water and adjust the pH to 2.5 with a pH meter using 1 0% H2 SO4 or NaOH. (ii) Di sodium EDTA solution: - Dissolve 0.73 gm of di sodium EDTA di hydrate in distilled water and make volume to 100 ml. B. Preparation of Standard stock solution of Magnesium 1000 ppm. - (i) Dissolve 1.0 gm of magnesium metal in 30 ml of 1:1 HCl and dilute to 1000 ml in volumetric flask with distilled water. This is 1000 ppm magnesium stock solution. (ii) Intermediate solution {100 ppm]. - Pipette 10 ml of magnesium stock solution and 20 ml of Disodium EDTA solution in 100 ml volumetric flask and dilute volume with distilled water. (Note: - In case the metal stock solution is not exactly I 00 ppm, the exact titer value of disodium EDTA required to chelate the metal has to be added.) (iii) Working Standard solution :- Pipette following volumes of 100 ppm intermediate solution in 100 ml Volumetric flask and make volume with acidified water. Volume of 100 ppm intermediate solut ion taken To make volume to 100 ml Concentration of Mg in ppm after making vol. to 100 ml 0.1 ml 100 ml 0.1 ppm 0.2 ml 100 m1 0.2 ppm 0.4 ml 100 m1 0.4 ppm 0.6 m1 100 m1 0.6 ppm C. Preparation of sample solution for Chelated Magnesium: - (i) Weigh 1.0 gm of sample and transfer into 100 ml volumetric flask and make volume with distilled water. Pipette out 2.0 ml of sample solution and dilute further to make second dilution of 100 ml with distilled water, pipette 1.0 ml from second dilution and make volume to 1 00 ml with acidified water to get the above flaming range from 0.1-0.25 ppm. (ii) Flaming the solutions: - Flame the standards and the sample of Chelated Magnesium on atomic absorption spectrophotometer at a wavelength of 285.2 rim (Mg line of instrument ) using air-acetylene. (iii) Prepare a blank solution from which only the sample has been omitted. Flame along with standards and sample. D. Calculations: - Prepare a standard curve of known concentration of Magnesium solutions by plotting the absorbance value on Y-axis against their respective concentrations on X-axis. Part -I :- Determination of Total Magnesium content in Chelated Magnesium sample. - Total Mg content = Concentration of Mg in ppm obtained after flaming the solution X 50 Part -II - Determination of Free magnesium in Chelated sample: - (i) Reagents: - 0.02 M Disodium salt of EDTA — 0.7444 gm of Disodium Salt of EDTA in 100 ml of distilled water. Standardize with 0.02 M standard Zinc and Manganese Metal solution. (ii) Buffer solution of pH — 10.0 (iii) Indicator Eriochrome black T (EBT) powder (iv) Take 10 ml of aliquot from stock dilution of sample (1.0 g in 100 ml) Add to it 0.1 gram Hydroxylamine HCl, 1to 2 ml of triethanolamine, 10 ml buffer of pH 10.0, EBT indicator and titrate against 0.02 M disodium EDTA solution. % Free Mag nesium=Titration value of EDTA x Atomic weight of Mg metal x Molarity of EDTA x 1 % Chelated Magnesium content = % Total Magnesium - % Free Magnesium content 39. Determination of Chelated Copper as Cu-EDTA: - A. Reagents: - (i) Glass distilled water or deminera lized water of pH 2.5+/ - 0.5:- Dilute l mI of 10% H2SO4 to one litre with glass distilled water or demineralized water and adjust the pH to 2.5 with a pH meter using 10% H2SO4 or NaOH. (ii) Di sodium EDTA solution: - Dissolve 0.73 gm of di sodium EDTA di hydrate in distilled water and make volume to 100 ml. B. Preparation of Standard stock solution of Copper 1000 ppm. - . (i) Dissolve 1.0 gm of Copper turnings in 30 ml of 1:1HNO3 and dilute to 1000 ml in volumetric flask with distilled water.This is 1000 ppm copper stock solution. (ii) Intermediate solution [100 ppm]. -Pipette 10 ml of Copper stock solution and 10 ml of Disodium EDTA solution in 100 ml volumetric flask and dilute volume with distilled water. (Note: - In case the metal stock solution is not exactly 100.0 ppm, the exact titer value of disodium EDTA required to chelate the metal has to be added.) (iii) Working Standard solution :- Pipette following volumes of 100 ppm intermediate solution in 100 ml Volumetric flask and make volume with acidified water. Volume of 100 ppm intermediate solution taken To make volume to 100 ml Concentration of Cu in ppm after making voI. to 100 ml 0.5 ml 100 ml 0.5 ppm 1.0 ml 100 ml 1. 0 ppm 1.5 ml 100 ml 1.5 ppm 2.0 ml 100 in1 2.0 ppm C. Preparation of sample solution for Chelat ed Copper: - (i) Weigh 1.0 gm of sample and transfer into 200 ml volumetric flask and make volume with distilled water. Pipette out 2.0 ml of sample solution and dilute further to make second dilution of 100 m l with distilled water, pipette 10.0 ml from second dilution and make volume to 100 ml with acidified water to get between the above flaming range from 0.5-2.0 ppm. (ii) Flaming the solutions: - Flame the standards and the sample of Chelated Copper on atomic absorption spectrophotometer at a wavelength of 324.8 nm (Cu line of instrument ) using air-acetylene flame. (iii) Prepare a blank solution from which only the sample has been omitted. Flame along with standards and sample. D. Calculations: - Prepare a standard curve of known concentration of Copper solutions by plotting the absorbance value on Y-axis against their respective concentrations on X-axis. Part -I :- Determination of Total Copper content in Chelated Copper sample: - Total Cu content = Concentration of Cu in ppm obtained after flaming the solution X 10 Part -II :- Determination of Free Copper in Chelated sample: - (i) Reagents: - 0.02 M Disodium salt of EDTA — 0.7444 gm of Disodium Salt of EDTA in 100 ml of distilled water. Standardize with 0.02 M standard Zinc/Manganese Metal solution. (ii) Acetate buffer (pH= 2.8) — 4.0 gm of Sodium acetate trihydrate + glacial acetic acid 155 ml make volume to 1000 ml. (iii) Methanolic PAN Indicator: - 0.2 gm of PAN indicator in 10 ml of methanol. Take 20 ml of aliquot from stock dilution of sample (1.0 g in 200 ml). Add 10 ml acetate bu ffer of pH 2.8 and PAN indicator solution and titrate against 0.02 M disodium EDTA solution. End point is Magenta red to yellowish green. % Free Copper=Titration value of EDTA x Atomic weight of Cu metal x Molarity of EDTA x 1 % Chelated Copper content = % Total Copper - % Free Copper content 40 Estimation of Zinc Content in Chelated Zinc as Zinc Glycine by AAS D. Principle: - The total Zinc in Zinc-Glycine is extracted by wet digestion (as Zinc) and estimated by using Atomic Absorption Spectrophotometer. I. Instruments / Equipments / Glasswares: - (i) Atomic Absorption Spectrophotometer; (ii) Hot Plate; (iii) Analytical Balance weight least count 0.1 mg; and (iv) Required Glasswares. 2. Reagents and Chemicals: - i) Zinc Metal (pure); ii) Nitric Acid (AR Grade conc.); iii) Sulphuric Acid (AR Grade conc.); iv) Perchloric Acid (AR Grade – 70%); and v) Tri Acid Mixture (10 part of Nitric Acid + 01 part of Sulphuric Acid + 04 parts of Perchloric Acid). 3. Procedure :- (a) Preparation of Standard Solutions - (i) Preparation of 1000 ppm Standard Solution of Zinc - Weigh accurately 500 mg of Zinc metal and dissolve in 10 ml conc. Hydrochloric Acid and make the volume to the volume of 500 ml with distilled water; and (ii) Preparation of Working Standard Solution – Take 1 ml, 2ml, 5 ml, 8 ml, 10 ml aliqu ot of 100 ppm standard solution in different 100 ml volumetric flasks and add 2 ml nitric acid in each volumetric flask and dilute to volume using distilled water. (iii) These solutions will be of 1.0, 2.0, 5.0, 8.0 and 10.0 ppm respectively. (b) Preparation of Sample Solution. - (i) Take 1.0 gm sample of Zinc Glycine weighed in 250 ml Erlenmeyer (Conical) flask; (ii) Add 10 ml of Tri acid mixture (10 part of Nitric acid : 1 part of Sulphuric acid : 4 parts of Perchloric acid); (iii) Boil the solution at temperature between 150 to 200o C on hot plate till the solution is clear and reduce the volume till white fumes appears; (iv) Then remove the solution from hot plate, cool it and make up the volume to 250 ml with distilled water; (v) Take 5 ml aliquot in 250 ml volumetric flask and make the volume with distilled water; and (vi) Run blank solution also to the same level. (c) Estimation of Zinc in the sample by AAS. – Flame the standard solution followed by sample solution at 215.9 nm wavelength. 4. Calculation: - Zinc % by weight :- (S-B) x V x D x 100 / W x 106 Where S = Average of concentration in sample reading in ppm V = Volume of solution B = Blank reading in ppm D = Dilution W = Weight of the sample in gm Note: - Distilled water instead of Milli -Q-Water has been used during analysis of the sample. 41. Estimation of Calcium Content in Chelated Calcium as Calcium Glycine by Atomic Absorption Spectrophotometer. Principle: - The total Calcium in Calcium -Glycine is extracted by wet digestion (as Calcium) and estimated by using Atomic Absorption Spec trophotometer. I. Instruments / Equipments / Glasswares: - (i) Atomic Absorption Spectrophotometer; (ii) Hot Plate; (iii) Analytical Balance weight least count 0.1 mg; and (iv) Required Glasswares. II. Reagents and Chemicals: - (i) Calcium Carbonate (Assay 99.5%); (ii) Nitric Acid (AR Grade conc.); (iii) Hydrochloric Acid (Assay 35.8 – 36.5%); (iv) Perchloric Acid (AR Grade – 70%); (v) Lanthanum Oxide (Assay 99.0%); (vi) Acid Mixture (Nitric Acid 2 parts : Perchloric Acid 1 part) ; and (vii) Lanthanum Oxide Solution: 5.86%w/v (58.65 gm of lanthanum oxide, 2 50 ml Hydrochloric acid and make up the volume 1000 ml with Milli -Q water). III. Procedure : A. Preparation of Standard Solutions. - (i) Preparation of 1000 ppm Standard Solution of Calcium - Weigh accurately 2.50 gm of Calcium Carbonate and dissolve in 1000 ml volumetric flask and make the volume to 1000 ml with distilled water; and (ii) Preparation of Working Standard Solution – Take 0.5 ml, 1.0 ml, 2.0 ml, 3.0 ml, 4.0 ml, 5 ml aliquot of 100 ppm standard solution in different 100 ml volumetric flasks and add 10 ml of lanthanum oxide solution in each volumetric flask and dilute to volume using distilled water. These solutions will be of 0.5, 1.0, 2.0, 3.0, 4.0, 5.0 ppm respectively. B. Preparation of Sample Solution. - (i) Take 1.0 gm sample of Calcium Glycine weighed in 250 ml Conical flask; (ii) Add 10 ml of acid mixture; (iii) Boil the solution at temperature between 150 to 200o C on hot plate till the solution is clear & reduce the volume till white fumes appears; (iv) Then remove the solution from hot plate, cool it and add 5 ml Hydroch loric acid and make up the volume to 250 ml with distilled water; (v) Take 2 ml aliquot in 250 ml volumetric flask and add 25 ml lanthanum oxide solution and make the volume with distilled water; and (vi) Run blank solution also to the same level. C. ESTIMATION of Calcium in the sample by AAS. – Flame the standard solution followed by sample solution at 422.67 nm wavelength. Calculation: - Calcium % by weight :- (S-B) x V x D x 100 / W x 106 Where S = Average of concentration in sample reading in ppm V = Volume of solution B = Blank reading in ppm D = Dilution W = Weight of the sample in gm Note: - Distilled water instead of Milli -Q-Water has been used during analysis of the sample. 5. In Schedule III, “PART -D, Method Of Analysis of Biofertilisers” in sub -heading “1(E) . Method of Analysis for Mycorhizal Biofertilisers,” in serial 3 “Estimation of total viable spores” for para (b),the following shall be substituted namely: - “(b) Procedure (i) Mix 100 gram Mycorrhizal biofertiliser in a substantial volum e of water and decant through a series of sieves arranged in descending order of mesh size; (ii) Vigorous washing with water is necessary to free spores from aggregates of clay carrier material or organic materials; (iii) Roots and coarse debris are collected on coar se sieves (1mm and 450 micron),while spores are captured on all the respective finer sieves (250 micron, 100 micron and 40/50 micron); (iv) Collect the sieving in jars and view the aliquots for spore count during continuous stirring on magnetic stirrer so that proper separation of spores and carrier could occur (as per suggestions from TERI experts); (v) Transfer aliquots from all the finer sieves (250 micron 100 micron and 40/50micron) are mixed and observed properly on the gridded Petri dishes or plate under stere omicroscope for total viable spore count; and (vi) Count the number of spores in plate/dish and express it as spores or gram of the sample. 6 A. In Schedule VII, under heading “GENERAL SPECIFICATIONS OF NANO FERTILISERS ” in sub-heading “1. Nano Nitrogen ”, in table, - (a) in serial” number 1, and entries relating thereto, shall be omitted; (b) in serial” number 4, and entries relating thereto, shall be omitted; (c) in serial” number 5, and entries relating thereto, shall be omitted; B. after serial number 2, “Na n o Ni tro g e n a nd P ho sp h o ru s Fe rt il iz er ”, and entries relating thereto, the following serial number and entries shall be inserted , namely, - “3 Nano Phosphorus fertilizer Sl. No. Parameters Specifications (i) Particle Size in nano meter (nm) in one direction: (ii) (a) Hydrodynamic particle Size (as per DLS ) (At least 50 % 0f the particles in the tested samples should be in range fulfilling the Department of Biotechnology guidelines) <100 nm (b) Physical Particle Size as per TEM analysis (At least 50 % 0f the particles in the tested samples should be in range fulfilling the Department of Biotechnology guidelines) <100nm (iii) Zeta Potential in ± mV(scale) 14to17 [F. No.-2-3/2023Fert Law] YOGITA RANA , Jt. Secy . Note: - The principal notification was publishe d in the Gazette of India, Extraordinary, Part II, section 3, sub -section (ii), vide notification GSR No.758 ( E) dated 25th September,1985 and was last amended vide notification number S.O. 1024 ( E) dated 2nd March, 2023. Uploaded by Dte. o f Printing at Government of India Press, Ring R oad, Mayapuri, New Delhi -110064 and Published by the Controller of Publications, Delhi -110054.